֪RcY
YǰһrεČWrMһȫϵyĿYʹ҂Ч҂һWYô֪YΌСʹij֪RcYgӭxϣ܉ϲg

֪RcY 1
(wĴ̼aһDŽӑB^)cm(ԣҲoBmֲıoɫ)ɻzQ
﹤̰֣քeǻ̡:(μg)lø
Ĺͬ|A(Ԫ)NNRNADNAнYʽY(Ǿa^;a^)zܴa
ԪغռrĶٵքeOCHNPSԪC
wПoC}ĹܺãȱF¼t\ݔ½wFSּ;ȱFؚѪwFSwΘɏs
ֲ:е|քeǣ֬|;:еҪ|Ҫ֬͵|^ֱԴҪԴԴԴ
|Yԭ(4)DNAYԭ(3)DNAYԭ(3)
СˮƽR¿ɿֱС0.2ļYСļ֧ԭw
|ĻԪCHONSԪ;ĻԪCHONPPԪ;ѪtԪCHONFe~GصԪCHONMgԪCHON;V|Ԫص֬
ԭ˼cТٟoĤʢڟoȾɫwۃHкwܼڳɷľǢzѭҎɢރHеĿz׃ǻͻ׃ߟoĤϵyYa^Bm;to˺;wMПoȡĤĺòϡ
|WĤϵycĤBcӺĤB߀cwĤB|MۯBMbǻȼӹγɾĤС\ݔߠwMһӹͷ
ڵпwɔ_(ǵ)øԭȍuءL^ĤԼYЃ|WߠwͼĤ
Nк˻Ҫȏƽ|SCȷֻŷֳɼzͼ|zɷNʽvδֻֻ˥ѴΔԽļԽL˥̓ͬõĽY
ֻČ|ǻxԱ_DˮƽɻɂȷǾa^{صġ
ȫָѷֻĵļаlĝ:ȫԴСɷ֞ȫԼ(̥)(ԭc)(Ѫɼ);ֲ:_ȫԴСǣֳܾw;ȫԵ|AǼȺбNȫz|
ӰøٷٶȵøضAȵʹø׃ԵǏᡢAߜwøĻԲضӰ-ømض60ҡ
̵ĹøԃøDNABø(cI);̵ĹøwSøzø(@ԭ|wrm˝ȵҺCȝBoԭ|w)ȵø(:)
ATPǼֱԴ|ڼȺ٣cADPDܵ\ݔźͷտs늡ЙCϳɵ;ܵНBv;γATPǺúⷴ
D׃ɻSWܕrӹwˮKwoø(NADP+)هB~GaˮNADPHγɵăɂʽ
߹ʵķ1LÕrg(һ݆)2ӹe(ֲg)3߹Ч(ٶ)
B܄(ˮͪƾ)ͨ^ĤĔUɢȑQĦǰٷ֝
vˮ\ݔˮֵĄҲ\ݔxӵĄ;ֲˮĄ߀Ǹ;Ӱvõǜضȡ(ض)Lֲˮ(1%--5%)vȴrFˮֲLĘ־
ҪֲͬͬˮͬMɲÇιMMйȣsˮ
oֳ(Ă)¡gֲMBֲָ̥gcDZHăԠֲMBăcǣȡBڶֳʸԄӻУٷֳ;oֲ;aˎʳƷӄɫءx;˹N;Dֲ
ֲˮֺ͌xӵǃɂ^עДٶȴС
w|āԴҪʳ֬ԴҪǸߵD
|wȲ܃ǼĽY||Դ|Óֽܷ|Ólڣ|z^ոz뵰||ֽ⡢^I
wÿ횔zһĵ|ԭǵ|ǼĽY||;|wȲ܃;DòγзNİ;|wÿ춼(횰ɫهKi)
^eȾɫwM(һͬԴȾɫw)ȾɫwM(wȵȾɫwN͔Ŀ)ȾɫwM(磺ѼȾɫwMɣ22l+X);Ի(ˮ24)ĆαM12lȾɫwzϢ;˻M(wDNAyȫzϢ)ˆαM(24lȾɫwzϢ);
ЙCxPϵ(ϵƼs)D(֬ܵ|);DЗl(ǹD׃֬ǿɴD׃ֻ֬֬D׃);Ƽs(ֻǴxϵKr֬|);ǴxĘм~
֪RcY 2
1.WfĽ^
(1)WfĄʼʩRǺʩ
(2)WfҪcǣһЙCwһЄֲﶼɼlɼͼa;һĆλԼcͬMɵw;¼ɏϼЮa
(3)WfĄMҪxǣʾκ΄ֲɼɵĶfֲ֮gһHPϵ֮gHPϵʾMҪrֵ
2.Nӵļ
(4)ȻϵyČӴУMϵywNȺȺBϵyȦ
(5)ֲϵyӴЛ]Сϵy@Ӵ
(6)ԭ˼c˼ı|^eПoԺĤļˡ
չ
ԭ˼w⣬oԭ缚ļҪɷc|Y϶ɵĻ
ԭzϵ zҎ;ֳ^˻zϵ zҎ
ȻlԭĿz׃ֻлͻ׃;Ŀz׃лͻ׃ؽMȾɫw׃
ԭ˼缚ҪԶѵķʽMз;˼ķѷʽнzѡozp
(7)ܪĴxͷֳ^ֻĻM
չ
ٲǼȲԭҲ
ڽMÿNĻλķNÓķNǺ
۲BֱBB鲡ķֳĻM
3.ĤϵyĽY
(8)ò.tܷxõļĤѼˮˮM뼚Ѽqƣȵ|@ӾͿԵõļĤ
(9)ĤҪ֬|͵|M߀
չ
ʹĤ|Mܵ|dw
ڼĤcĤĻWMɴͬڲͬĤW|ĺвe磬ĤĺcĤҪ
(10)ĤĽYcx^
(11)ڼĤһɼĤϵĵ|cY϶ɵǵDZDZcRePϵͺƤǵбo͝
(12)ֲ:ڵĻWɷҪwSغz
չ
ټڵijɷc|Y϶ɵĻ
ڳwSøzøȥֲ:
֪RcY 3
1zy߀ԭԇ0.1g/mlNaOHҺ0.05g/mlCuSO4ҺMc߀ԭǰlɴutɫʹÕrעFF
2bMе֬KȾҺKȾҺǰߌ֬ȾSɫȾɼtɫ
3bMеĵ|psԇʹÕrȼNaOHҺ2~3CuSO4Һɫj
4oȾɫwȾɫõȾɫđϻtҺ
5^DNARNAڼеķֲüG_tɷNȾɫȾɫDNAȾɾGɫRNAȾɼtɫ
6ԭ|ӰĤҺĤԼ@ɌĤ֮gļ|
7ǾGȾҺnjһȾwĻȾʹоwʬF{Gɫ
8ڷڵĺϳɡӹ\ݔͷ^УPļw|Wߠww
9γҪͨ^Óˮsϵķʽ
10ҺȴڼҺȕrֲ:l|ڷxF;ҺСڼҺȕrֲ:l|ڷxďԭF
֪RcY 4
һ WгҊWԪؼã
1Cawȱ֮ܛѪҺCa2+͕鴤^߄toѪҺеCa2+дMѪҺ̵ÙccѪҺеCa2+ѪҺͲlֲвٵԪһȱ۵ĽMܵ
2FeѪtĽMɳɷȱȱFؚѪѪtеFeǶrFrFDzõֲвٵԪһȱ۵ĽMܵ
3Mg~GwĽMԪܶøļֲȱVr~׳F~}ʧG
4BM۵Ȱlͻ۹ܵLȱֲF
5Iנټصijɷȱ׃СY˕طԼנ[
6KѪ⛺^͕rFļԄӹɮʧ
7NNǘ~GATP|ͺıԪNֲwγɵĻﶼDzĻˮNֲwȿƄȱNr~~N~SNһNˮBϵyIBһNWԪأˮBϵy^NcPϕɸIBڵˮBϵyеĸIBQ顰ˮAںBϵyеĸIBQ顰ೱwȱNHȱٰᣬ͕Ӱ푵wLl
8PPǘ֬ATPıԪءֲwȱPӰ푵DNAďƺRNAD䛣ĶӰ푵ֲLlP߀cֲúͺеf^ATPADPжᡣPҲˮBϵyIBһNԪֲȱPr~׳Fo~Gϼtɫt
9ZnijЩøĽMɳɷҲøĻġͽzϳɫøкZn]ZnͲܺϳᡣȱZnOҵֲС~Yͅ~Y~׃Сgs
Wгõԇ
1ԇ ɷ֣0.1g/ml NaOHҺ0.05g/ml CuSO4Һ÷ԇҺҺweٌϺԇyҺˮԡӟֱӼӟyҺд߀ԭtʴutɫ
2Ƕԇ{ɫҺǻϺˮԡFutɫǵĜy
3psԇɷ֣0.1g/ml NaOHҺ0.01g/ml CuSO4Һ÷yҺȼ2mlҺuм3~4Һuyдڵ|tʬFɫ
4K÷ȡKw95%ľƾУuڙzy֬Ɍ֬ȾSɫKȾɼtɫ
5bDNADNAˮԡȾ{ɫ
6GbDNADNAGأȾ{Gɫ
750%ľƾҺ֬bKȾҺȾɫ50%ľƾҺϴȥɫ
875%ľƾҺښ75%ľƾܝB뼚ʹ|׃ԡ@ƾĝBÓˮÜp@tʹ浰|ѸÓˮ̳ĤKƾ75ľƾҺgǰQˎᘾǰƤwÓԼCе
995%ľƾҺswe֔95%ľƾDNA
1015%}95%ľƾҺweϿڽx⡣
11đҺ(Ȟ0.01g/ml0.02g/ml)ȾɫwɫɌȾɫwȾɫͨȾɫ3~5ҲôtȾɫ
1220%ĸK3%^3.5%ȻFڱ^^øFe3+ĴЧrĸKк^ø
133%ĿԵҺ3%Һ2%røҺ̽øۺǵÌ
14Һb۵Ĵڡ׃{
15ͪȡ~Gwеɫء
16Һɷ֣20ʯ2ݱͪ1ݱ϶Ҳ93̖ͣɫصČɫڞVϷx_
17裺ɫصȡķxĥGɫ~Ƭrʹĥ֡
18̼}ĥGɫ~ƬrкЙCֹĥr~GwеɫƉ
190.3g/mLҺஔ30%Һֲ:Һĝȴ|ڷx
200.1g/mLęcҺcuѪϣѪ
21ȻcҺٿܽDNAȻcȞ2mol/L 0.015mol/LrDNAܽȻcȞ0.14 mol/LrDNAܽڝȞ0.9%r}ˮ
22ȵøٿÁֽ⵰|ڿڄ:BrֽMʹMɢ
23ˮأ˹TwԇȰlķNӻ磬ʹȾɫwMӱԭǿڷѵļNwγ
24Ȼ}Ӽڵͨԣڻ̵Dʹ̎ڸܑB
֪RcYСP£
αһ֪RcY02-19
Y03-16
tlj֪RcY12-12
tlj֪RcY12-11
γ֪RcYͨ13ƪ08-30
Z֪Rc֮ZR06-20
07-28
ˮGij֪Rc10-19
tlj֪Rc12-20