֪RcY

rg2022-09-14 12:02:31 Y

֪RcY

YǰһrεČWrMһȫϵyĿYʹ҂Ч׌҂һWYô֪YΌСʹij֪RcYgӭxϣ܉ϲg

֪RcY

֪RcY 1

(wĴ̼aһDŽӑB^)cm(ԣҲoBmֲıoɫ)ɻzQ

﹤̰󲿷֣քeǻ򹤳̡:(μg)l͹ø

Ĺ԰ͬ|A(Ԫ)NNRNADNAнYʽY(Ǿa^;a^)zܴa

ԪغռrĶٵքeOCHNPSԪC

wПoC}ĹܺãȱF¼t\ݔ½wFSּ;ȱFؚѪwFSwΘɏs

ֲ:е|քeǣ֬|;:еҪ|Ҫ֬͵|^ֱԴҪԴԴԴ

|Yԭ(4)DNAYԭ(3)DNAYԭ(3)

С΢ˮƽR¿ɿֱС0.2΢׵ļ΢YСļ֧ԭw

|ĻԪCHONSԪ;ĻԪCHONPPԪ;Ѫt׵ԪCHONFe~GصԪCHONMgԪCHON;V|Ԫص֬

ԭ˼cТٟoĤʢڟoȾɫwۃHкwܼڳɷľǢzѭҎɢރHеĿz׃ǻͻ׃ߟoĤϵyYa^Bm;to˺;wMПoȡĤĺòϡ

|WĤϵycĤBcӺĤB߀cwĤB|MۯBMbǻȼӹγɾĤС\ݔߠwMһӹͷ

ڵпwɔ_(ǵ)øԭȍuءL^ĤԼYЃ|WߠwͼĤ

Nк˻Ҫȏƽ|SCȷֻŷֳɼzͼ|zɷNʽvδֻֻ˥ѴΔԽļԽL˥̓ͬõĽY

ֻČ|ǻxԱ_DˮƽɻɂȷǾa^{صġ

ȫָѷֻĵļаlĝ:ȫԴСɷ֞ȫԼ(̥)(ԭc߼)(Ѫɼ);ֲ:_ȫԴСǣֳܾw;ȫԵ|AǼȺбNȫz|

ӰøٷٶȵøضAȵʹø׃ԵǏᡢAߜ؄wøĻԲضӰ-ømض60ҡ

򹤳̵ĹøԃøDNABø(cI);̵ĹøwSø͹zø(@ԭ|wrm˝ȵҺCȝBoԭ|w)ȵø(:)

ATPǼֱԴ|ڼȺ٣cADP໥Dܵ\ݔźͷտs񽛂늡ЙCϳɵ;ܵНB͸v;γATPǺú͹ⷴ

D׃ɻSWܕrӹwˮKwoø(NADP+)هB~GaˮNADPHγɵăɂʽ

߹ʵķ1LÕrg(һ݆)2ӹe(ֲg)3߹Ч(ٶ)

B͸܄(ˮͪƾ)ͨ^͸ĤĔUɢȑQĦǰٷ֝

vˮ\ݔˮֵĄҲ\ݔxӵĄ;ֲˮĄ߀Ǹ;Ӱvõǜضȡ(ض)Lֲˮ(1%--5%)vȴrFˮֲLĘ־

ҪֲͬͬˮͬMɲÇιMMйȣsˮ

oֳ(Ă)¡gֲMBֲָ̥gcDZHăԠֲMBăcǣȡBڶֳʸԄӻУٷֳ;oֲ;aˎʳƷӄɫءx;˹N;Dֲ

ֲˮֺ͌xӵǃɂ^עДٶȴС

w|āԴҪʳ֬ԴҪǸߵ׵D

|wȲ܃ǼĽY|͹|Դ|Óֽܷ|Ólڣ|z^ոz뵰||ֽ⡢^I

wÿ횔zһĵ|ԭǵ|ǼĽY|͹|;|wȲ܃;DòγзNİ;|wÿ춼(횰᣺ɫهKi)

^eȾɫwM(һ׷ͬԴȾɫw)ȾɫwM(wȵȾɫwN͔Ŀ)ȾɫwM(磺ѼȾɫwMɣ22l+X);Ի(ˮ24)ĆαM12lȾɫwzϢ;˻M(wDNAyȫzϢ)ˆαM(24lȾɫwzϢ);

ЙCxPϵ(໥“ϵ໥Ƽs)D(֬ܵ|);DЗl(ǹD׃֬ǿɴD׃ֻ֬֬D׃);໥Ƽs(ֻǴxϵKr֬|);ǴxĘм~

֪RcY 2

1.WfĽ^

(1)WfĄʼʩRǺʩ

(2)WfҪcǣһЙCwһЄֲﶼɼlɼͼa;һĆλԼ֌cͬMɵw;¼ɏϼЮa

(3)WfĄMҪxǣʾκ΄ֲɼɵĶfֲ֮gһHPϵ֮gHPϵʾMҪrֵ

2.Nӵļ

(4)ȻϵyČӴУMϵywNȺȺBϵyȦ

(5)ֲϵyӴЛ]Сϵy@Ӵ

(6)ԭ˼c˼ı|^eПoԺĤ޵ļˡ

չ

ԭ˼w⣬oԭ缚ļҪɷc|Y϶ɵĻ

ԭzϵ zҎ;ֳ^˻zϵ zҎ

ȻlԭĿz׃ֻлͻ׃;Ŀz׃лͻ׃ؽMȾɫw׃

ԭ˼缚ҪԶѵķʽMз;˼ķѷʽнzѡozp

(7)ܪĴxͷֳ^ֻĻM

չ

ٲǼȲԭҲ

ڽMÿNĻλķNÓķNǺ

۲BֱBB鲡ķֳĻM

3.ĤϵyĽY͹

(8)ò.tܷxõļĤѼˮˮM뼚Ѽqƣȵ|@ӾͿԵõļĤ

(9)ĤҪ֬|͵|M߀

չ

ʹĤ|Mܵ|dw

ڼĤcĤĻWMɴͬڲͬĤW|ĺвe磬ĤĺcĤҪ

(10)ĤĽYcx͸^

(11)ڼĤһɼĤϵĵ|cY϶ɵǵDZDZcRePϵͺƤǵбo͝

(12)ֲ:ڵĻWɷҪwSغ͹z

չ

ټڵijɷc|Y϶ɵĻ

ڳwSø͹zøȥֲ:

֪RcY 3

1zy߀ԭԇ0.1g/mlNaOHҺ0.05g/mlCuSO4ҺMc߀ԭǰlɴutɫʹÕrעFF

2bMе֬KȾҺKȾҺǰߌ֬ȾSɫȾɼtɫ

3bMеĵ|psԇʹÕrȼNaOHҺ2~3CuSO4Һɫj

4oȾɫwȾɫõȾɫđϻtҺ

5^DNARNAڼеķֲü׻G_tɷNȾɫȾɫDNAȾɾGɫRNAȾɼtɫ

6ԭ|ӰĤҺĤԼ@ɌĤ֮gļ|

7ǾGȾҺnjһȾwĻȾʹоwʬF{Gɫ

8ڷڵ׵ĺϳɡӹ\ݔͷ^УPļw|Wߠww

9γҪͨ^Óˮsϵķʽ

10ҺȴڼҺȕrֲ:l|ڷxF;ҺСڼҺȕrֲ:l|ڷxďԭF

֪RcY 4

һ WгҊWԪؼã

1Cawȱ֮ܛѪҺCa2+͕鴤^߄t𼡟oѪҺеCa2+дMѪҺ̵ÙccѪҺеCa2+ѪҺͲlֲвٵԪһȱ۵ĽMܵ

2FeѪt׵ĽMɳɷȱȱFؚѪѪtеFeǶrFrFDzõֲвٵԪһȱ۵ĽMܵ

3Mg~GwĽMԪܶøļֲȱVr~׳F~}ʧG

4BM۵Ȱlͻ۹ܵLȱֲF

5Iנټصijɷȱ׃СY˕طԼנ[

6KѪ⛺^͕rFļԄӹɮʧ

7NNǘ~GATP|ͺıԪNֲwγɵĻﶼDzĻˮNֲwȿƄȱNr~~N~SNһNˮBϵyIBһNWԪأˮBϵy^NcPϕɸIBڵˮBϵyеĸIBQ顰ˮAںBϵyеĸIBQ顰ೱwȱNHȱٰᣬ͕Ӱ푵wLl

8PPǘ֬ATPıԪءֲwȱPӰ푵DNAďƺRNAD䛣ĶӰ푵ֲLlP߀cֲúͺеf^ATPADPжᡣPҲˮBϵyIBһNԪֲȱPr~׳Fo~Gϼtɫt

9ZnijЩøĽMɳɷҲøĻġ߻ͽzϳɫøкZn]ZnͲܺϳᡣȱZnOҵֲС~Yͅ~Y~׃Сgs

Wгõԇ

1ԇ ɷ֣0.1g/ml NaOHҺ0.05g/ml CuSO4Һ÷ԇҺҺweٌϺԇyҺˮԡӟֱӼӟyҺд߀ԭtʴutɫ

2Ƕԇ{ɫҺǻϺˮԡFutɫǵĜy

3psԇɷ֣0.1g/ml NaOHҺ0.01g/ml CuSO4Һ÷yҺȼ2mlҺuм3~4Һuyдڵ|tʬFɫ

4K÷ȡKw95%ľƾУuڙzy֬Ɍ֬ȾSɫKȾɼtɫ

5bDNADNAˮԡȾ{ɫ

6׻GbDNADNA׻GأȾ{Gɫ

750%ľƾҺ֬bKȾҺȾɫ50%ľƾҺϴȥɫ

875%ľƾҺښ75%ľƾܝB뼚ʹ|׃ԡ@ƾĝB͸ÓˮÜp@tʹ浰|ѸÓˮ̳ĤKƾ͸75ľƾҺgǰQˎᘾǰƤwÓԼCе

995%ľƾҺswe֔95%ľƾDNA

1015%}᣺95%ľƾҺweϿڽx⡣

11đҺ(Ȟ0.01g/ml0.02g/ml)ȾɫwɫɌȾɫwȾɫͨȾɫ3~5ҲôtȾɫ

1220%ĸK3%^3.5%ȻFڱ^^øFe3+Ĵ߻ЧrĸKк^ø

133%ĿԵҺ3%Һ2%røҺ̽øۺǵÌ

14Һb۵Ĵڡ׃{

15ͪȡ~Gwеɫء

16Һɷ֣20ʯ2ݱͪ1ݱ϶Ҳ93̖ͣɫصČɫڞVϷx_

17裺ɫصȡķxĥGɫ~Ƭrʹĥ֡

18̼}ĥGɫ~ƬrкЙCֹĥr~GwеɫƉ

190.3g/mLҺஔ30%Һֲ:Һĝȴ|ڷx

200.1g/mLęcҺcuѪϣѪ

21ȻcҺٿܽDNAȻcȞ2mol/L 0.015mol/LrDNAܽȻcȞ0.14 mol/LrDNAܽڝȞ0.9%r}ˮ

22ȵøٿÁֽ⵰|ڿڄ:BrֽMʹMɢ

23ˮأ˹T౶wԇȰlķNӻ磬ʹȾɫwMӱԭǿڷѵļNwγ

24Ȼ}Ӽڵͨ͸ԣڻ򹤳̵Dʹ̎ڸܑB

֪RcYСP£

αһ֪RcY02-19

Y03-16

tlj֪RcY12-12

tlj֪RcY12-11

γ֪RcYͨ13ƪ08-30

Z֪Rc֮ZR06-20

07-28

ˮGij֪Rc10-19

tlj֪Rc12-20